Application

Western blot: 1-10 µg/mL using ECL. Recognizes a 57kDa band representing the H1 receptor

ELISA: 1:10,000-1:100,000 using 50-100 ng control peptide per well.

Optimal working dilutions must be determined by the end user.

This Anti-Histamine Receptor 1 Antibody, C-terminus is validated for use in ELISA, WB for the detection of Histamine Receptor 1.

Research CategoryInflammation & Immunology

Research Sub CategoryInflammation & Autoimmune Mechanisms

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

A 17 amino acid peptide sequence near the cytoplasmic, C-terminus of rat HR1 (Fujimoto et al. 1993; Inoue et al. 1996; DeBacker et al. 1993; Fukui et al. 1994; Moguilevsky et al. 1994).

Epitope: C-terminus

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Liquid in PBS containing 0.1% BSA.

Specificity

Recognizes rat Histamine Receptor 1 (HR1). The immunogen shows no significant sequence homology with other histamine receptors.

The immunogen peptide is 100% conserved in mouse and bovine, 94% in human, fish and monkey and 80% in guinea pig. Reactivity with other species has not been confirmed.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.